The International Mouse Phenotyping Consortium (IMPC) is a major international effort to explore the effects of knocking out 20,000 genes in the mouse. A new study by White and colleagues, published in the current issue of Disease Models & Mechanisms, demonstrates the usefulness of lacZ in situ reporter expression patterns in extending our understanding of genotype-phenotype relationships as pa...

EPITHELIUM-FIBRE CELL COUPLING IN THE EYE LENS PRESCOTT, A.R., DAHM. R.. HUTCHESON, A.M., QUIN!-AN. R.A., VANMARLE. J.‘, & VRENSEN. G§. Department of Biochemistry, University of Dundee, Medical Sciences Institute, Dundee, DDl 4HN. Scotland. * Department of Electron Microscopy, Faculty of Medicine, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. 5 Department of Morp...

In Alzheimer disease, increased b-secretase (BACE1) activity has been associated with neurodegeneration and accumulation of amyloid precursor protein (APP) products. Thus, inactivation of BACE1 could be important in the treatment of Alzheimer disease. In this study, we found that lowering BACE1 levels using lentiviral vectors expressing siRNAs targeting BACE1 reduced amyloid production and the ...

Summary: Protein identification by mass spectrometry is commonly accomplished using a peptide sequence matching search algorithm, whose sensitivity varies inversely with the size of the sequence database and the number of post-translational modifications considered. We present the Spectrum Identification Machine, a peptide sequence matching tool that capitalizes on the high-intensity b1-fragmen...